This study highlights the importance of entrepreneurial culture in educational institutions as an organization. This study emphasizes the relationship between entrepreneurial culture, entrepreneurship education, and entrepreneurial intention as study variables. This study highlights several aspects: the relationship between variables, study trend, contextual aspect like (country or educational institutions), population, publication type, and study design. A scoping review method is considered for this study based on both Scopus and Web of Science databases. The covered publications in the analyses were for the last 20 years ranging from the year 2003 till August 2021, with no limits on languages. After filtration, the eligible number of studies was (n = 94) out of 180 from both databases. The methodology part contains several sections based on the PRISM-ScR checklist. The findings show a significant relationship among the three variables with the possibility of entrepreneurial culture being a mediator between entrepreneurship education and entrepreneurial intention for future studies, which has not been tested yet. The highest publications werein 2015, especially by European Union countries, as studies in developed countries were more than developing and economies in transition countries. Quantitative studies were more than qualitative and mixed methods, as studies considered HEIs more than schools focusing on students and then teachers. To conclude, policymakers are encouraged to follow policies to improve entrepreneurial culture growth by promoting strategic cooperation among stakeholders and educational institutions implementing several concepts to enhance entrepreneurial culture. Future studies should validate and test these variables’ relationships through a standard and accurate instrument.